Journal: Transplant international : official journal of the European Society for Organ Transplantation

Article Title: Safety and pharmacodynamics of anti-CD2 monoclonal antibody treatment in cynomolgus macaques

doi: 10.1111/tri.13524

Figure Lengend Snippet: Animal characteristics and treatment regimens

Article Snippet: The test substances were manufactured to target the CD2 antigen, and included two modifications of the same anti-CD2 mAb: a rat anti-primate CD2 IgG2b (Immerge BioTherapeutics) (hereafter termed RT-CD2), and the further modified rhesus recombinant anti-primate CD2 IgG1 (hereafter termed RH-CD2).

Techniques:

Journal: Transplant international : official journal of the European Society for Organ Transplantation

Article Title: Safety and pharmacodynamics of anti-CD2 monoclonal antibody treatment in cynomolgus macaques

doi: 10.1111/tri.13524

Figure Lengend Snippet: A) WBC counts in all treated animals, groups A-C (n.s.). B, C) Showing treatment impact on hematocrit (Hct%) or platelet levels (n.s.). D) ALT and AST levels pre- and post-anti-CD2 infusion, group A. E, F) Post-treatment levels of leukocyte subsets. G) Showing bimodal CD2 and CD3 T cell expression in naïve cynomolgus monkeys, with memory cells having a higher CD2hi and CD3lo expression. Shown is a representative gate defining the bimodal distribution, and the three subsets of T cells (TN, TEM, TCM) gated for CD2 and CD3. Predominantly, TN are CD3hiCD2lo and memory cells CD3loCD2hi. CCR7/CD45RA gating step not shown. H) Average median fluorescence intensities (MFI) of cell surface CD2 on CD4+-, CD8+-, NK-, B-, and regulatory T cells from naïve animals (n=4). I) Showing partial competitive binding of RH-CD2 and CD2-BV786 antibodies. J, K) RH-CD2 effectively depletes memory cells, while relatively sparing naïve lymphocytes in group A. L) Illustrating the CD8/CD4 ratio before and after RH-CD2 in group A. M) Showing a 695% CD25hiFoxP3+ cell enrichment from day 0 to day 7 after RH-CD2 infusion (p=0.052) in group A. Data is presented as average (±SEM). Abbreviations: WBC, white blood cell count; CD, cluster of differentiation; CM, central memory; EM, effector memory; NK cell, natural killer cell.

Article Snippet: The test substances were manufactured to target the CD2 antigen, and included two modifications of the same anti-CD2 mAb: a rat anti-primate CD2 IgG2b (Immerge BioTherapeutics) (hereafter termed RT-CD2), and the further modified rhesus recombinant anti-primate CD2 IgG1 (hereafter termed RH-CD2).

Techniques: Expressing, Fluorescence, Binding Assay, Cell Counting

Journal: Transplant international : official journal of the European Society for Organ Transplantation

Article Title: Safety and pharmacodynamics of anti-CD2 monoclonal antibody treatment in cynomolgus macaques

doi: 10.1111/tri.13524

Figure Lengend Snippet: The allogeneic MLRs were inhibited by varying concentrations of RT- and RH-CD2, respectively, at the onset of the host effector cell cultures. After four days, MLRs were pulsed for 24 hours with 1μCi 3H-thymidine per well. The cellular radioactivity was measured in triplicates samples. Statistical analysis were performed of antibody and no antibody treatments using one-way ANOVA. (A) RT-CD2 (B) RH-CD2.

Article Snippet: The test substances were manufactured to target the CD2 antigen, and included two modifications of the same anti-CD2 mAb: a rat anti-primate CD2 IgG2b (Immerge BioTherapeutics) (hereafter termed RT-CD2), and the further modified rhesus recombinant anti-primate CD2 IgG1 (hereafter termed RH-CD2).

Techniques: Radioactivity

Journal: Transplant international : official journal of the European Society for Organ Transplantation

Article Title: Safety and pharmacodynamics of anti-CD2 monoclonal antibody treatment in cynomolgus macaques

doi: 10.1111/tri.13524

Figure Lengend Snippet: Effector cells stably transfected with A) FcγRI (n=3), B) FcγRIIA (n=3) or C) FcγRIIIA (n=2) and a luciferase reporter gene were incubated with serial dilutions of antibody. Upon binding of a target-bound IgG antibody (the reporter cells expressed CD2) luciferase expression was induced. Luminescence values were normalized to the highest concentration of the positive control. RH-CD2 displayed clear dose-dependent FcγR-mediated signaling but RT-CD2 did not. D) Both antibodies induced dose-dependent CDC of lymphocytes relative to untreated controls (n=4).

Article Snippet: The test substances were manufactured to target the CD2 antigen, and included two modifications of the same anti-CD2 mAb: a rat anti-primate CD2 IgG2b (Immerge BioTherapeutics) (hereafter termed RT-CD2), and the further modified rhesus recombinant anti-primate CD2 IgG1 (hereafter termed RH-CD2).

Techniques: Stable Transfection, Transfection, Luciferase, Incubation, Binding Assay, Expressing, Concentration Assay, Positive Control